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| Author: |
Hacek, D. M., Trick, W. E., Collins, S. M., Noskin, G. A. and Peterson, L. R. |
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| Title |
Comparison of the rodac imprint method to selective enrichment broth for recovery of vancomycin-resistant enterococci and drug-resistant enterobacteriaceae from environmental surfaces |
| Source |
Journal of Clinical Microbiology, 38(12): 4646-4648 |
| Citation:
Hacek, D. M., Trick, W. E., Collins, S. M., Noskin, G. A. and Peterson, L. R., (2000), "Comparison of the rodac imprint method to selective enrichment broth for recovery of vancomycin-resistant enterococci and drug-resistant enterobacteriaceae from environmental surfaces", Journal of Clinical Microbiology, 38(12): 4646-4648.
| | | Notes: |
| We compared the Rodac imprint technique to selective enrichment broth for detecting vancomycin-resistant enterococci (VRE) and multidrug-resistant Enterobacteriaceae (MDRE) on surfaces. Rodac plates contained tryptic soy agar with 5% sheep blood, vancomycin (6 µg/ml), ceftazidime (2 µg/ml), amphotericin B (2 µg/ml), and clindamycin (1 µg/ml). Two types of broth were used: brain heart infusion (BHI) and BHI plus vancomycin (6 µg/ml) and ceftazidime (2 µg/ml) (BHIVC). Of the 46 surfaces cultured for VRE, 12 (26%) were positive. Of the 12 VRE-positive surfaces, 11 (92%) grew from Rodac, 8 (67%) grew from BHIVC, and 7 (58%) grew from BHI. A larger study is needed for MDRE, as only 4 of 43 surfaces were MDRE positive. The Rodac imprint technique successfully recovered VRE from environmental surfaces. |
This publication in whole or part may be found online at:  http://jcm.asm.org/cgi/content/full/38/12/4646 |
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